rna iscript reverse transcriptase Search Results


tris hcl thermo fisher 15 568 025 rnase outtm thermo fisher 10777019 iscript advanced reaction mix  (Thermo Fisher)
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Tris Hcl Thermo Fisher 15 568 025 Rnase Outtm Thermo Fisher 10777019 Iscript Advanced Reaction Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Timeline for profiling MSN subtype translatomes in fentanyl abstinence. Male and female D1- and A2A-Cre mice were crossed with Cre-dependent ribosome tagged mice (Rpl22HA; RiboTag), then underwent homecage fentanyl drinking and abstinence (n=6 samples/sex/cell-type/drug, 4 mice pooled per sample). ( B ) HA-tagged ribosomes from D1- or D2-MSNs were immunoprecipitated and purified mRNA was used for Nanostring or to prepare <t>cDNA</t> libraries for RNAseq. Cell-type specific RNAseq data were analyzed by Weighted Gene Co-expression Network Analysis (WGCNA). ( C ) Top: number of genes that exhibited a nominally significant effect (p<0.05) of fentanyl, sex, or cell type. Bottom: number of genes with nominally significant effect of fentanyl in D1- and D2-MSNs showing little overlap between subtypes. ( D ) Clustering dendrograms from analysis of the 4591 genes in D1- and D2-MSNs with WGCNA. Module colors are shown for modules pertaining to fentanyl abstinence, defined as differential module eigengene expression with p<0.05 effect of fentanyl abstinence. ( E ) Circos plot showing 11 selected WGCNA modules as arbitrary colors (outermost ring), ranked clockwise by overall fold change in D1-MSNs. Fold change in eigengene expression, and –log 10 ( P value) in D1- and D2-MSNs are shown internally. ( F ) Network structure of the green module. Hub genes are encircled in green, and the 3 hub genes selected for Nanostring are also encircled in yellow. Hub gene circle sizes are scaled based on the number of connections ( G ) Circos plot showing Nanostring analysis of 3 hub genes for each WGCNA module (outermost segments). Inner rings show fentanyl vs water differential expression scaled by –log 10 ( P value) with significant ( P <0.05) differences featured in color. The outer comparison rings show data with males and females combined, while the pink and blue encircled rings show comparisons in females or males only. See also Fig S3, Table S1. Detailed statistics in Supplemental File 1.
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(A) Timeline for profiling MSN subtype translatomes in fentanyl abstinence. Male and female D1- and A2A-Cre mice were crossed with Cre-dependent ribosome tagged mice (Rpl22HA; RiboTag), then underwent homecage fentanyl drinking and abstinence (n=6 samples/sex/cell-type/drug, 4 mice pooled per sample). ( B ) HA-tagged ribosomes from D1- or D2-MSNs were immunoprecipitated and purified mRNA was used for Nanostring or to prepare <t>cDNA</t> libraries for RNAseq. Cell-type specific RNAseq data were analyzed by Weighted Gene Co-expression Network Analysis (WGCNA). ( C ) Top: number of genes that exhibited a nominally significant effect (p<0.05) of fentanyl, sex, or cell type. Bottom: number of genes with nominally significant effect of fentanyl in D1- and D2-MSNs showing little overlap between subtypes. ( D ) Clustering dendrograms from analysis of the 4591 genes in D1- and D2-MSNs with WGCNA. Module colors are shown for modules pertaining to fentanyl abstinence, defined as differential module eigengene expression with p<0.05 effect of fentanyl abstinence. ( E ) Circos plot showing 11 selected WGCNA modules as arbitrary colors (outermost ring), ranked clockwise by overall fold change in D1-MSNs. Fold change in eigengene expression, and –log 10 ( P value) in D1- and D2-MSNs are shown internally. ( F ) Network structure of the green module. Hub genes are encircled in green, and the 3 hub genes selected for Nanostring are also encircled in yellow. Hub gene circle sizes are scaled based on the number of connections ( G ) Circos plot showing Nanostring analysis of 3 hub genes for each WGCNA module (outermost segments). Inner rings show fentanyl vs water differential expression scaled by –log 10 ( P value) with significant ( P <0.05) differences featured in color. The outer comparison rings show data with males and females combined, while the pink and blue encircled rings show comparisons in females or males only. See also Fig S3, Table S1. Detailed statistics in Supplemental File 1.
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(A) Timeline for profiling MSN subtype translatomes in fentanyl abstinence. Male and female D1- and A2A-Cre mice were crossed with Cre-dependent ribosome tagged mice (Rpl22HA; RiboTag), then underwent homecage fentanyl drinking and abstinence (n=6 samples/sex/cell-type/drug, 4 mice pooled per sample). ( B ) HA-tagged ribosomes from D1- or D2-MSNs were immunoprecipitated and purified mRNA was used for Nanostring or to prepare <t>cDNA</t> libraries for RNAseq. Cell-type specific RNAseq data were analyzed by Weighted Gene Co-expression Network Analysis (WGCNA). ( C ) Top: number of genes that exhibited a nominally significant effect (p<0.05) of fentanyl, sex, or cell type. Bottom: number of genes with nominally significant effect of fentanyl in D1- and D2-MSNs showing little overlap between subtypes. ( D ) Clustering dendrograms from analysis of the 4591 genes in D1- and D2-MSNs with WGCNA. Module colors are shown for modules pertaining to fentanyl abstinence, defined as differential module eigengene expression with p<0.05 effect of fentanyl abstinence. ( E ) Circos plot showing 11 selected WGCNA modules as arbitrary colors (outermost ring), ranked clockwise by overall fold change in D1-MSNs. Fold change in eigengene expression, and –log 10 ( P value) in D1- and D2-MSNs are shown internally. ( F ) Network structure of the green module. Hub genes are encircled in green, and the 3 hub genes selected for Nanostring are also encircled in yellow. Hub gene circle sizes are scaled based on the number of connections ( G ) Circos plot showing Nanostring analysis of 3 hub genes for each WGCNA module (outermost segments). Inner rings show fentanyl vs water differential expression scaled by –log 10 ( P value) with significant ( P <0.05) differences featured in color. The outer comparison rings show data with males and females combined, while the pink and blue encircled rings show comparisons in females or males only. See also Fig S3, Table S1. Detailed statistics in Supplemental File 1.
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(A) Timeline for profiling MSN subtype translatomes in fentanyl abstinence. Male and female D1- and A2A-Cre mice were crossed with Cre-dependent ribosome tagged mice (Rpl22HA; RiboTag), then underwent homecage fentanyl drinking and abstinence (n=6 samples/sex/cell-type/drug, 4 mice pooled per sample). ( B ) HA-tagged ribosomes from D1- or D2-MSNs were immunoprecipitated and purified mRNA was used for Nanostring or to prepare <t>cDNA</t> libraries for RNAseq. Cell-type specific RNAseq data were analyzed by Weighted Gene Co-expression Network Analysis (WGCNA). ( C ) Top: number of genes that exhibited a nominally significant effect (p<0.05) of fentanyl, sex, or cell type. Bottom: number of genes with nominally significant effect of fentanyl in D1- and D2-MSNs showing little overlap between subtypes. ( D ) Clustering dendrograms from analysis of the 4591 genes in D1- and D2-MSNs with WGCNA. Module colors are shown for modules pertaining to fentanyl abstinence, defined as differential module eigengene expression with p<0.05 effect of fentanyl abstinence. ( E ) Circos plot showing 11 selected WGCNA modules as arbitrary colors (outermost ring), ranked clockwise by overall fold change in D1-MSNs. Fold change in eigengene expression, and –log 10 ( P value) in D1- and D2-MSNs are shown internally. ( F ) Network structure of the green module. Hub genes are encircled in green, and the 3 hub genes selected for Nanostring are also encircled in yellow. Hub gene circle sizes are scaled based on the number of connections ( G ) Circos plot showing Nanostring analysis of 3 hub genes for each WGCNA module (outermost segments). Inner rings show fentanyl vs water differential expression scaled by –log 10 ( P value) with significant ( P <0.05) differences featured in color. The outer comparison rings show data with males and females combined, while the pink and blue encircled rings show comparisons in females or males only. See also Fig S3, Table S1. Detailed statistics in Supplemental File 1.
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(A) Timeline for profiling MSN subtype translatomes in fentanyl abstinence. Male and female D1- and A2A-Cre mice were crossed with Cre-dependent ribosome tagged mice (Rpl22HA; RiboTag), then underwent homecage fentanyl drinking and abstinence (n=6 samples/sex/cell-type/drug, 4 mice pooled per sample). ( B ) HA-tagged ribosomes from D1- or D2-MSNs were immunoprecipitated and purified mRNA was used for Nanostring or to prepare <t>cDNA</t> libraries for RNAseq. Cell-type specific RNAseq data were analyzed by Weighted Gene Co-expression Network Analysis (WGCNA). ( C ) Top: number of genes that exhibited a nominally significant effect (p<0.05) of fentanyl, sex, or cell type. Bottom: number of genes with nominally significant effect of fentanyl in D1- and D2-MSNs showing little overlap between subtypes. ( D ) Clustering dendrograms from analysis of the 4591 genes in D1- and D2-MSNs with WGCNA. Module colors are shown for modules pertaining to fentanyl abstinence, defined as differential module eigengene expression with p<0.05 effect of fentanyl abstinence. ( E ) Circos plot showing 11 selected WGCNA modules as arbitrary colors (outermost ring), ranked clockwise by overall fold change in D1-MSNs. Fold change in eigengene expression, and –log 10 ( P value) in D1- and D2-MSNs are shown internally. ( F ) Network structure of the green module. Hub genes are encircled in green, and the 3 hub genes selected for Nanostring are also encircled in yellow. Hub gene circle sizes are scaled based on the number of connections ( G ) Circos plot showing Nanostring analysis of 3 hub genes for each WGCNA module (outermost segments). Inner rings show fentanyl vs water differential expression scaled by –log 10 ( P value) with significant ( P <0.05) differences featured in color. The outer comparison rings show data with males and females combined, while the pink and blue encircled rings show comparisons in females or males only. See also Fig S3, Table S1. Detailed statistics in Supplemental File 1.
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(A) Timeline for profiling MSN subtype translatomes in fentanyl abstinence. Male and female D1- and A2A-Cre mice were crossed with Cre-dependent ribosome tagged mice (Rpl22HA; RiboTag), then underwent homecage fentanyl drinking and abstinence (n=6 samples/sex/cell-type/drug, 4 mice pooled per sample). ( B ) HA-tagged ribosomes from D1- or D2-MSNs were immunoprecipitated and purified mRNA was used for Nanostring or to prepare <t>cDNA</t> libraries for RNAseq. Cell-type specific RNAseq data were analyzed by Weighted Gene Co-expression Network Analysis (WGCNA). ( C ) Top: number of genes that exhibited a nominally significant effect (p<0.05) of fentanyl, sex, or cell type. Bottom: number of genes with nominally significant effect of fentanyl in D1- and D2-MSNs showing little overlap between subtypes. ( D ) Clustering dendrograms from analysis of the 4591 genes in D1- and D2-MSNs with WGCNA. Module colors are shown for modules pertaining to fentanyl abstinence, defined as differential module eigengene expression with p<0.05 effect of fentanyl abstinence. ( E ) Circos plot showing 11 selected WGCNA modules as arbitrary colors (outermost ring), ranked clockwise by overall fold change in D1-MSNs. Fold change in eigengene expression, and –log 10 ( P value) in D1- and D2-MSNs are shown internally. ( F ) Network structure of the green module. Hub genes are encircled in green, and the 3 hub genes selected for Nanostring are also encircled in yellow. Hub gene circle sizes are scaled based on the number of connections ( G ) Circos plot showing Nanostring analysis of 3 hub genes for each WGCNA module (outermost segments). Inner rings show fentanyl vs water differential expression scaled by –log 10 ( P value) with significant ( P <0.05) differences featured in color. The outer comparison rings show data with males and females combined, while the pink and blue encircled rings show comparisons in females or males only. See also Fig S3, Table S1. Detailed statistics in Supplemental File 1.
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(A) Timeline for profiling MSN subtype translatomes in fentanyl abstinence. Male and female D1- and A2A-Cre mice were crossed with Cre-dependent ribosome tagged mice (Rpl22HA; RiboTag), then underwent homecage fentanyl drinking and abstinence (n=6 samples/sex/cell-type/drug, 4 mice pooled per sample). ( B ) HA-tagged ribosomes from D1- or D2-MSNs were immunoprecipitated and purified mRNA was used for Nanostring or to prepare <t>cDNA</t> libraries for RNAseq. Cell-type specific RNAseq data were analyzed by Weighted Gene Co-expression Network Analysis (WGCNA). ( C ) Top: number of genes that exhibited a nominally significant effect (p<0.05) of fentanyl, sex, or cell type. Bottom: number of genes with nominally significant effect of fentanyl in D1- and D2-MSNs showing little overlap between subtypes. ( D ) Clustering dendrograms from analysis of the 4591 genes in D1- and D2-MSNs with WGCNA. Module colors are shown for modules pertaining to fentanyl abstinence, defined as differential module eigengene expression with p<0.05 effect of fentanyl abstinence. ( E ) Circos plot showing 11 selected WGCNA modules as arbitrary colors (outermost ring), ranked clockwise by overall fold change in D1-MSNs. Fold change in eigengene expression, and –log 10 ( P value) in D1- and D2-MSNs are shown internally. ( F ) Network structure of the green module. Hub genes are encircled in green, and the 3 hub genes selected for Nanostring are also encircled in yellow. Hub gene circle sizes are scaled based on the number of connections ( G ) Circos plot showing Nanostring analysis of 3 hub genes for each WGCNA module (outermost segments). Inner rings show fentanyl vs water differential expression scaled by –log 10 ( P value) with significant ( P <0.05) differences featured in color. The outer comparison rings show data with males and females combined, while the pink and blue encircled rings show comparisons in females or males only. See also Fig S3, Table S1. Detailed statistics in Supplemental File 1.
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(A) Timeline for profiling MSN subtype translatomes in fentanyl abstinence. Male and female D1- and A2A-Cre mice were crossed with Cre-dependent ribosome tagged mice (Rpl22HA; RiboTag), then underwent homecage fentanyl drinking and abstinence (n=6 samples/sex/cell-type/drug, 4 mice pooled per sample). ( B ) HA-tagged ribosomes from D1- or D2-MSNs were immunoprecipitated and purified mRNA was used for Nanostring or to prepare <t>cDNA</t> libraries for RNAseq. Cell-type specific RNAseq data were analyzed by Weighted Gene Co-expression Network Analysis (WGCNA). ( C ) Top: number of genes that exhibited a nominally significant effect (p<0.05) of fentanyl, sex, or cell type. Bottom: number of genes with nominally significant effect of fentanyl in D1- and D2-MSNs showing little overlap between subtypes. ( D ) Clustering dendrograms from analysis of the 4591 genes in D1- and D2-MSNs with WGCNA. Module colors are shown for modules pertaining to fentanyl abstinence, defined as differential module eigengene expression with p<0.05 effect of fentanyl abstinence. ( E ) Circos plot showing 11 selected WGCNA modules as arbitrary colors (outermost ring), ranked clockwise by overall fold change in D1-MSNs. Fold change in eigengene expression, and –log 10 ( P value) in D1- and D2-MSNs are shown internally. ( F ) Network structure of the green module. Hub genes are encircled in green, and the 3 hub genes selected for Nanostring are also encircled in yellow. Hub gene circle sizes are scaled based on the number of connections ( G ) Circos plot showing Nanostring analysis of 3 hub genes for each WGCNA module (outermost segments). Inner rings show fentanyl vs water differential expression scaled by –log 10 ( P value) with significant ( P <0.05) differences featured in color. The outer comparison rings show data with males and females combined, while the pink and blue encircled rings show comparisons in females or males only. See also Fig S3, Table S1. Detailed statistics in Supplemental File 1.
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(A) Timeline for profiling MSN subtype translatomes in fentanyl abstinence. Male and female D1- and A2A-Cre mice were crossed with Cre-dependent ribosome tagged mice (Rpl22HA; RiboTag), then underwent homecage fentanyl drinking and abstinence (n=6 samples/sex/cell-type/drug, 4 mice pooled per sample). ( B ) HA-tagged ribosomes from D1- or D2-MSNs were immunoprecipitated and purified mRNA was used for Nanostring or to prepare <t>cDNA</t> libraries for RNAseq. Cell-type specific RNAseq data were analyzed by Weighted Gene Co-expression Network Analysis (WGCNA). ( C ) Top: number of genes that exhibited a nominally significant effect (p<0.05) of fentanyl, sex, or cell type. Bottom: number of genes with nominally significant effect of fentanyl in D1- and D2-MSNs showing little overlap between subtypes. ( D ) Clustering dendrograms from analysis of the 4591 genes in D1- and D2-MSNs with WGCNA. Module colors are shown for modules pertaining to fentanyl abstinence, defined as differential module eigengene expression with p<0.05 effect of fentanyl abstinence. ( E ) Circos plot showing 11 selected WGCNA modules as arbitrary colors (outermost ring), ranked clockwise by overall fold change in D1-MSNs. Fold change in eigengene expression, and –log 10 ( P value) in D1- and D2-MSNs are shown internally. ( F ) Network structure of the green module. Hub genes are encircled in green, and the 3 hub genes selected for Nanostring are also encircled in yellow. Hub gene circle sizes are scaled based on the number of connections ( G ) Circos plot showing Nanostring analysis of 3 hub genes for each WGCNA module (outermost segments). Inner rings show fentanyl vs water differential expression scaled by –log 10 ( P value) with significant ( P <0.05) differences featured in color. The outer comparison rings show data with males and females combined, while the pink and blue encircled rings show comparisons in females or males only. See also Fig S3, Table S1. Detailed statistics in Supplemental File 1.
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Image Search Results


(A) Timeline for profiling MSN subtype translatomes in fentanyl abstinence. Male and female D1- and A2A-Cre mice were crossed with Cre-dependent ribosome tagged mice (Rpl22HA; RiboTag), then underwent homecage fentanyl drinking and abstinence (n=6 samples/sex/cell-type/drug, 4 mice pooled per sample). ( B ) HA-tagged ribosomes from D1- or D2-MSNs were immunoprecipitated and purified mRNA was used for Nanostring or to prepare cDNA libraries for RNAseq. Cell-type specific RNAseq data were analyzed by Weighted Gene Co-expression Network Analysis (WGCNA). ( C ) Top: number of genes that exhibited a nominally significant effect (p<0.05) of fentanyl, sex, or cell type. Bottom: number of genes with nominally significant effect of fentanyl in D1- and D2-MSNs showing little overlap between subtypes. ( D ) Clustering dendrograms from analysis of the 4591 genes in D1- and D2-MSNs with WGCNA. Module colors are shown for modules pertaining to fentanyl abstinence, defined as differential module eigengene expression with p<0.05 effect of fentanyl abstinence. ( E ) Circos plot showing 11 selected WGCNA modules as arbitrary colors (outermost ring), ranked clockwise by overall fold change in D1-MSNs. Fold change in eigengene expression, and –log 10 ( P value) in D1- and D2-MSNs are shown internally. ( F ) Network structure of the green module. Hub genes are encircled in green, and the 3 hub genes selected for Nanostring are also encircled in yellow. Hub gene circle sizes are scaled based on the number of connections ( G ) Circos plot showing Nanostring analysis of 3 hub genes for each WGCNA module (outermost segments). Inner rings show fentanyl vs water differential expression scaled by –log 10 ( P value) with significant ( P <0.05) differences featured in color. The outer comparison rings show data with males and females combined, while the pink and blue encircled rings show comparisons in females or males only. See also Fig S3, Table S1. Detailed statistics in Supplemental File 1.

Journal: bioRxiv

Article Title: Negative emotional behavior during fentanyl abstinence is mediated by adaptations in nucleus accumbens neuron subtypes

doi: 10.1101/2022.05.15.491856

Figure Lengend Snippet: (A) Timeline for profiling MSN subtype translatomes in fentanyl abstinence. Male and female D1- and A2A-Cre mice were crossed with Cre-dependent ribosome tagged mice (Rpl22HA; RiboTag), then underwent homecage fentanyl drinking and abstinence (n=6 samples/sex/cell-type/drug, 4 mice pooled per sample). ( B ) HA-tagged ribosomes from D1- or D2-MSNs were immunoprecipitated and purified mRNA was used for Nanostring or to prepare cDNA libraries for RNAseq. Cell-type specific RNAseq data were analyzed by Weighted Gene Co-expression Network Analysis (WGCNA). ( C ) Top: number of genes that exhibited a nominally significant effect (p<0.05) of fentanyl, sex, or cell type. Bottom: number of genes with nominally significant effect of fentanyl in D1- and D2-MSNs showing little overlap between subtypes. ( D ) Clustering dendrograms from analysis of the 4591 genes in D1- and D2-MSNs with WGCNA. Module colors are shown for modules pertaining to fentanyl abstinence, defined as differential module eigengene expression with p<0.05 effect of fentanyl abstinence. ( E ) Circos plot showing 11 selected WGCNA modules as arbitrary colors (outermost ring), ranked clockwise by overall fold change in D1-MSNs. Fold change in eigengene expression, and –log 10 ( P value) in D1- and D2-MSNs are shown internally. ( F ) Network structure of the green module. Hub genes are encircled in green, and the 3 hub genes selected for Nanostring are also encircled in yellow. Hub gene circle sizes are scaled based on the number of connections ( G ) Circos plot showing Nanostring analysis of 3 hub genes for each WGCNA module (outermost segments). Inner rings show fentanyl vs water differential expression scaled by –log 10 ( P value) with significant ( P <0.05) differences featured in color. The outer comparison rings show data with males and females combined, while the pink and blue encircled rings show comparisons in females or males only. See also Fig S3, Table S1. Detailed statistics in Supplemental File 1.

Article Snippet: Purified RNA was quantified with a Nanodrop (Thermo), then 500ng of complementary DNA (cDNA) was synthesized using the reverse transcriptase iScript complementary DNA synthesis kit (Bio-Rad, Hercules, CA; # 1708891), then diluted to a concentration of 2.5 ng/μL.

Techniques: Immunoprecipitation, Purification, Expressing, Quantitative Proteomics, Comparison